D-葡萄糖[HK法]检测试剂盒

High purity reagents for the assay of D-glucose in plant and food products. Can be used in combination with other Megazyme products that require glucose determination. Content:110 assays per kit

UV-method for the determination of D-Glucose in foodstuffs,
beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

Kit size:  (K-GLUHKR)
 110 assays (manual) / 1100 (microplate) 
 / 1000 (auto-analyser) or
 (K-GLUHKL)
 220 assays (manual) / 2200 (microplate) 
 / 2000 (auto-analyser)
Method:  Spectrophotometric at 340 nm
Reaction time:  ~ 5 min
Detection limit:  0.66 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals
(e.g. infusions), feed, paper (and cardboard) and other materials (e.g.
biological cultures, samples, etc.)
Method recognition: 
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years after preparation
  
• Rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Extended cofactors stability
  
• Suitable for manual, microplate and auto-analyser formats