乳糖检测试剂盒

Content: 65 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Lactose

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 340

Signal Response: Increase

Linear Range: 1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)

Limit of Detection: 1.62 mg/L

Reaction Time (min): ~ 10 min

Method recognition: AOAC Method 2020.08

The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.

Advantages

World’s first sequential assay for lactose, i.e. improves accuracy and efficiency 

Contains a specific β-galactosidase for the selective measurement of lactose in dairy products 

Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products 

Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1.62 mg/L 

Very competitive price (cost per test)

内容：每个试剂盒65次检测

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：乳糖

测定形式：分光光度计

检测方法：吸光度

波长（nm）：340

信号响应：增加

线性范围：1至50µg乳糖（或0.50至25µg D-葡萄糖）

检出限：1.62 mg/L

反应时间（分钟）：~10分钟

方法识别：AOAC方法2020.08

K-LOLAC检测试剂盒可快速、新颖、连续地测量传统低乳糖和无乳糖乳制品中的游离葡萄糖和乳糖。与传统的乳糖测定格式相比，这种顺序测定格式减少了分析员所需的手动输入，从而提高了准确性和效率。当与提供的Megazyme蠕变计算器结合使用时，该试剂盒中使用的β-半乳糖苷酶可以在低聚半乳糖（GOS）存在的情况下选择性测量乳糖，低聚半乳糖通常存在于无乳糖乳制品中。这是对现有商业上可获得的乳糖检测试剂盒的显著改进，由于β-半乳糖苷酶对GOS的非选择性水解，该试剂盒通常高估了无乳糖样品中的乳糖含量。最后，通过使用级联生化途径，K-LOLAC检测试剂盒的灵敏度提高了一倍，有助于显著降低检测的LOD和LOQ。

优点

世界上第一个乳糖连续测定法，即提高准确性和效率

含有一种特定的β-半乳糖苷酶，用于选择性测量乳制品中的乳糖

高效的预处理步骤可准确测量“低乳糖”和“无乳糖”乳制品中的乳糖

检测下限（LOD）比任何其他市售的酶促乳糖检测方法都低。1.62 mg/L时的LOD

极具竞争力的价格（每次测试的成本）