Megazyme 低聚-α-1,6-葡萄糖苷酶加β-半乳糖苷酶

Shipping Temperature: Ambient

Storage Temperature: 4oC

Formulation: In 3.2 M ammonium sulphate

Physical Form: Suspension

Stability: > 3 years at 4oC

Enzyme Activity: Oligo-1,6-α-Glucosidase, β-Galactosidase

EC Number: oligo-α-1,6-glucosidase: 3.2.1.10

β-galactosidase: 3.2.1.23

CAZy Family: GH13, GH35

CAS Number: oligo-α-1,6-glucosidase: 9032-15-9

β-galactosidase: 9013-11-2

Synonyms: oligo-α-1,6-glucosidase: oligosaccharide 6-alpha-glucohydrolase

β-galactosidase: beta-D-galactoside galactohydrolase

Source: oligo-α-1,6-glucosidase: Microbial

β-galactosidase: Aspergillus niger

Molecular Weight: oligo-α-1,6-glucosidase: 67,300

β-galactosidase: 125,000

Concentration: Oligo-α-1,6-glucosidase (2,000 U/mL) / β-galactosidase (2,000 U/mL)

Expression: Oligo-α-1,6-glucosidase: Recombinant from a Microbial source

β-Glucosidase: Purified from Aspergillus niger

Specificity: This enzyme mixture gives complete hydrolysis of isomaltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf.

Specific Activity: Oligo-α-1,6-glucosidase (~ 170 U/mg) / β-galactosidase (~ 170 U/mg)

Unit Definition: Oligo-α-1,6-glucosidase: One Unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40oC.

β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.

Temperature Optima: 50oC

pH Optima: 4.5

Application examples: Used for removal of isomaltose and lactose in the measurement of dietary fiber.