Bioclone 5μm BcMag long-arm NHS-activated ?magnetic beads SKU: FI-107

货号:FI-107

规格:5ml (30mg/ml)

报价:3576.00

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商品描述

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BcMag™ NHS-Activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with high density NHS (N-
hydroxyl succinimide) functional groups on the surface.  The beads are used to specifically conjugate primary Amine-containing
ligands (Fig.1), so other active groups in the ligand do not need to be protected prior to coupling. Coupling is fast (15–30 min at
room temp pH 6.5–9, 4 hours at 4 °C).  BcMag™ NHS-activated Magnetic Beads are most suitable for conjugation of  large
proteins. BcMagTM Long-arm (17 atome) NHS-activated Magnetic Beads are recommended for conjugation of small peptides
because the long-arm (21-atom) hydrophilic linker may reduce steric hindrance.

Features and Advantages:

  • Pre-activated and ready-to-use
  • Quick coupling in neutral pH (6.5–9) 4 to 25°C.
  • Stable covalent bond with minimal ligand leakage
  • Produces reusable immunoaffinity matrices
  • Low nonspecific binding
  • Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
  • Applications: Cell sorting, Immunoprecipitation; Purification
     for Antibodies, Proteins/Peptides, DNA/RNA
Product specificities
Composition
Silica-coated iron oxide magnetic beads  grafted with  NHS group on the surface
Bead Size
1µm diameter;  ~5µm diameter
Number of Beads
~1.7 x 108 beads/mg;   ~5 x 107 beads  (5µm beads) /mg
Surface Area
~100 m2/g
Magnetization
~40 EMU/g
Type of Magnetization
Superparamagnetic
Effective Density
2.5 g/ml
Stability
pH 4-10;Temperature:  4°C -140°C; Most organic solvents
Concentration
30 mg/ml in 100%  isopropanol
Functional Group Density
1µm Magnetic Beads
~250 µmole / g of Beads
5µm  Magnetic Beads
~200 µmole / g of Beads
1µm Long-Arm -Magnetic Beads
~210 µmole / g of Beads
5µm Long-Arm Magnetic Beads
~170 µmole  / g of Beads
Storage
Store at 4°C from light upon receipt
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